25-Hydroxy Vitamin-D Test

What is the 25-Hydroxy Vitamin D Test?

Vitamin D is one of the essential vitamins. It is necessary for our body. It helps in absorbing calcium and maintaining the health of strong bones. Our body can naturally produce vitamin D when our skin comes in contact with the sun. Other common sources of vitamin D include eggs, fish, and even fortified dietary products. 

However, before your body starts to use vitamin D, it needs to undergo various processes. The liver is the first center where it is transformed. In the liver, vitamin D is converted into calcidiol or 25-hydroxyvitamin D. 

This test is also known as the calcium 25-hydroxycholecalciferol or 25-OH vitamin D test. It is an essential indicator of bone malformation (rickets), bone weakness (osteoporosis), and osteomalacia.

Vitamin D test in Pakistan is a routine test that helps medical practitioners to get to know the level of vitamin D in your body.

Vitamin D Test Price in Pakistan

25 Hydroxy Vitamin D test price in Pakistan ranges between (1000-4000) Rupees. The Vitamin D test price in Pakistan varies depending upon the lab and city you are choosing to get your test. However, to get a discount on Vit d test price in Karachi, Lahore, Islamabad, Rawalpindi, Multan, and other major cities, you can book your Vitamin D test from Healthwire.


How can I Book Vitamin D Test Near Me?

The 25-Hydroxy Vitamin D test is a special test that is performed on a daily basis in the labs. You can easily book a Vitamin D test online. For efficient service and affordable Vitamin D test price in Lahore, Karachi, Islamabad, Rawalpindi, and other major cities, you can rely on Healthwire. You can call us at 042352500989/02137130261 or click on the test for instant booking of the Vitamin D test.

To book your 25-Hydroxy Vitamin D test:

  • You can simply click on the lab test online via our web healthwire.pk or Healthwire app 
  • Select the 25-Hydroxy vitamin D test or upload prescription
  • Pay in cash or online (debit/credit card, jazzcash)
  • Our representative will call you for confirmation

You can also call us at 042352500989  or click on the test for instant booking. You can also avail discount on the Vitamin D test cost if you book your test via Healthwire.

Why is a 25-Hydroxy Vitamin D Test Conducted?

This test is recommended by your doctor for many reasons such as:

  • To evaluate if too low or too much level of vitamin D in the body is causing abnormalities or any bone weakness (softness, weakness, or even fracture)
  • To diagnose people who are at risk or suffering from vitamin D deficiency
  • Before starting the treatment of osteoporosis
  • To monitor the efficacy of treatment in patients who are intaking vitamin D, phosphorus, calcium, and magnesium supplements
  • To monitor or diagnose the problem related to the PTH (parathyroid hormone
  • To properly monitor the overall health of the patients especially those with Crohn’s disease, fibrosis, or those who had just undergone bypass surgery

It is a routine test that is conducted on a daily basis in the labs of Lahore, Karachi, Multan, Rawalpindi, Islamabad, and other major cities

How to Interpret the 25-Hydroxy Vitamin D Test?

The results of the vitamin D test vary depending upon the gender, age testing method, and the lab that you are opting for the test. 

The level of vitamin D is measured by taking into account the reference range. 

If the level of vitamin D is low, it is due to either decreased absorption of vitamin D from the intestine or less exposure to the sun. While the higher level would be due to excess intake of vitamin D supplements. 

The normal range of the vitamin D is measured in nanograms per milliliter (ng/mL) or in nmol/L or it can also vary depending upon the vitamin d test lab in Lahore, Karachi, Multan, Islamabad, and other major cities. 


Normal Range (Reference Range ng/mL)


Less than 12 ng/mL (30 nmol/L)

Potential Deficiency

Between (12-20)ng/mL or (30-50) nmol/L

Normal Levels

(20-50)ng/mL or (50-125) nmol/L

High Levels

Higher value than 50 (ng/mL) or 125 nmol/L


Book this lab test?

25-Hydroxy Vitamin-D Test

Rs. 2664.0 Rs. 3700

Frequently Asked Questions (FAQs)

Sub Total
Rs. 3700 Rs. 2664.0